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Introducing Deep Purple Total Protein Stain - the new solution to sharpen your image

.. Introduction More Volume: Comparison of Deep Purple and Sypro Ruby in 1D PAGE Less Noise: Comparison of Deep Purple and Sypro Ruby in 2D PAGE Ettan DIGE System and MS Compatibility Now you can use Deep Purple Total Protein Stain to stain blots as well as gels Technical Specification Ordering Information (order online today) . Click here for more information Click here for more information Introduction Visualization of proteins separated by gel electrophoresis has traditionally been performed using CoomassieTM blue or silver staining. However, fluorescent detection offers substantial advantages over these colorimetric methods such as increased sensitivity, simple robust staining protocols, and quantitative reproducibility over a broad dynamic range. Deep PurpleTM Total Protein Stain offers superior 1-D and 2-D gel image data by delivering higher sensitivity and clearer backgrounds compared to other fluorescent stains such as SyproTM Ruby. Deep Purple can be detected using a broad variety of imaging devices including industry standard fluorescent scanners such as TyphoonTM, CCD cameras, UV transilluminators and some light boxes. The stain is fully compatible with mass spectrometry (MS) and Edman sequencing. As Deep Purple is a naturally occurring fluorophore free from heavy metals, it is easily disposed of and environmentally friendly. Deep Purple simply gives you more volume with less noise providing up to eight times more sensitivity than Sypro Ruby with no ‘speckling’. . Up to eight times more sensitive than Sypro Ruby The most sensitive method available today No speckling Clearer, easily discernible, and more accurately quantitated protein spots and bands Low background High signal-to-noise ratios means low-intensity spots and bands are detected Flexible detection Compatible with most fluorescent scanners and CCD cameras, UV transilluminators, and light boxes Naturally occurring fluorophore free from heavy metals Easy to dispose of and environmentally friendly Low viscosity Easier to handle with no oily residue Compatible with downstream analysis Fully compatible with MS and Edman sequencing Top More Volume: Comparison of Deep Purple and Sypro Ruby in 1D PAGE . Albumin (1A and 1B) and phosphorylase b (2A and 2B) loaded as doubling dilutions starting from 512 ng and separated according to a standard 1-D gel electrophoresis protocol then stained with Deep Purple (1A and 2A) and Sypro Ruby (1B and 2B). Scanned using Typhoon 9410 Variable Mode Imager.Volumes of each of the bands were calculated using ImageQuantTM software (background corrected) and plotted against protein load giving graphs 1C for albumin and 2C for phosphorylase b. Deep Purple exhibits both an advantage in terms of sensitivity (up to eight-fold improvement) and in terms of intensity. For full experimental details click here.(pdf) Top Less Noise: Comparison of Deep Purple and Sypro Ruby in 2D PAGE ... 2 2-D gels of a protein sample consisting of a mix of HBL100 breast cell line and BT474 breast cell carcinoma stained with (A) Sypro Ruby and (B) Deep Purple. For clarity, the gel images show pH 3–8 where most of the proteins are present. The expanded region of the gel stained with Sypro Ruby (gel A) and resulting 3-D plot demonstrate the drawbacks associated with ‘speckling’. Staining with Deep Purple (gel B) eliminates speckling and improves spot clarity, which allows more accurate spot detection and protein identification. First dimension: pH 3–10 NL 24cm ImmobilineTM DryStrip strip run on EttanTM IPGphorTM II IEF System; second dimension: 12.5% SDS electrophoresis gel run on Ettan DALTtwelve electrophoresis system. Scanned using Typhoon 9410 Variable Mode Imager. For full experimental details click here.(pdf) Top Ettan DIGE System and MS Compatibility .. Deep Purple is compatible with the standard Ettan DIGE system workflow and fully compatible with downstream analysis such as MALDI-TOF MS. The figure shows images of CyDyeTM DIGE fluor-labelled analytical gels (CyTM2, Cy3, and Cy5), and an image of a poststained Deep Purple preparatory gel with highlighted spots that were picked for MS analysis using Ettan MALDI-ToF Pro. Proteins were correctly identified as vimentin (pI 5.0, Mr 53 700) (A) and isocitrate dehydrogenase (pI 6.2, Mr 46 000) (B). Note the clarity of the spectra. For full experimental details click here.(pdf) Top Now you can use Deep Purple Total Protein Stain to stain blots as well as gels Easy-to-use Rapid protocol (30 min), simple (five short steps) and robust Sensitive The most sensitive fluorescent blot stain available. Sixteen times more sensitive than Sypro™ Ruby blot stain, low background and high intensity signal Versatile Use with PVDF or nitrocellulose membranes, scan using UVA or UVB epi-illumination, or 488 nm/532 nm lasers, and blots can be stained in wet or dry form Compatible Use with subsequent immunological analysis, Edman sequencing and mass spectrometry Quantitative The linear response is only limited by how much protein can be quantitatively blotted to membrane (typically two orders of magnitude) For full experimental details on Deep Purple™ Total Protein Stain and Sypro Ruby blot stain comparison click here. Top Technical Specifications Supplied as a 200 x concentrate for storage convenience Shipped and stored at -20 °C Stable for up to one week once reconstituted at +4 °C Stable for at least three months at -20 °C Excitation maxima: 528 nm Emission maxima: 594 nm Top Order now:

Deep Purple Total Protein Stain is exclusively licensed to Amersham Biosciences from FluoroTechnics Pty Ltd.
Deep Purple Total Protein Stain may only be used for applications in life science research.